Journal: Physiological Research

Article Title: Analysis of Gene Expression Profiles Regulating Phenotypic Transformation of Vascular Smooth Muscle Cells by Endothelial Cell-Derived Exosomes

doi: 10.33549/physiolres.935541

Figure Lengend Snippet: A ) TEM (×30,000, voltage = 100.0 kV) reveals that ECs exosomes have a double-concave disc structure. B ) NTA showed an average diameter of 85.45 nm, with 99.33 % of the exosomes at this size, consistent with typical extracellular vesicles (30–150 nm). C )WB analysis confirmed high expression of exosome marker proteins CD9, CD63, and CD81 in ECs exosomes.

Article Snippet: The complete medium for rat aortic ECs ( Procell Life Science & Technology Co., Ltd” (Wuhan, China, CM-R075)), it contained 5 % FBS, 50ng/mL VEGF, 10ng/mL EGF, 20ng/mL bFGF and 1 % penicillin and streptomycin,CD9, CD31, CD63, and CD81 monoclonal antibodies (Abcam, USA), fluorescein (Cy3)-labeled goat anti-rabbit IgG (Boster, Wuhan, China), exosome extraction kit (exoEasy Maxi Kit, Qiagen), GW4869 exosome inhibitor (Yensen, China), SW-CJ-IF clean bench, constant temperature CO 2 incubator (Thermo, USA), 6-well Transwell culture plate (0.4 mm) (Costar 3450, USA), Olympus inverted microscope, fluorescence microscope, Agilent 2100 Bioanalyzer (Agilent Technologies, USA), and NovaSeq 6000 (Illumina, USA) were employed in this investigation.

Techniques: Expressing, Marker